The increased of MMP-9 and MMP-2 with the decreased of TIMP-1 the uterosacral ligament after childbirth

Abstract

Introduction: pelvic floor dysfunction is one of the causes of morbidity that have very active role in the degradation of collagen and elastin through an intermediary changes in matrix metalloproteinases (MMP) and its regulation. This research to evaluate level of MMP-9, MMP-2 and TIMP-1 expression on uterosacral ligament in women after vaginal delivery. Methods: this research was conducted by collecting samples in consecutive sampling of biopsy uterosacral ligament from primigravida who underwent cesarean section because of obstructive labor at stage 1 and 2. As control, biopsy samples were taken from uterosacral ligament of primigravida a term who have not entered the labor phase and undergoing elective caesarean section surgery. The method in this study was cross sectional. The examination of MMP-9, MMP-2, and TIMP-1 expression by immunohistochemistry based on comparison of the sample and the control differences. Results: there are differences in the levels of Matrix Metalloproteinase-9, Matrix Metalloproteinase-2, and TIMP-1 in the uterosacral ligament of primigravida women in labor compared with women who are never in labor. Conclusion: there are increased levels of Matrix Metalloproteinase-9 and Matrix Metalloproteinase-2 with the decreased of TIMP-1 in the uterosacral ligament of primigravida women who underwent cesarean section because of obstructive labor at stage 1 and 2.