Talin anchors and nucleates actin filaments at lipid membranes A direct demonstration

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Abstract

Platelet talin nucleates actin assembly as we show here directly by using rhodamine-phalloidin labelling of actin filaments, Nucleation by talin still occurs after reconstitution into liposomal bilayers. This is also demonstrated directly after protein-lipid double labelling and light microscopic imaging. Talin, thus, is the first actin binding protein for which anchoring and nucleation of artin filament growth at lipid interfaces have been visualized. © 1992.

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Kaufmann, S., Käs, J., Goldmann, W. H., Sackmann, E., & Isenberg, G. (1992). Talin anchors and nucleates actin filaments at lipid membranes A direct demonstration. FEBS Letters, 314(2), 203–205. https://doi.org/10.1016/0014-5793(92)80975-M

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