Cyclic strain alters the expression and release of angiogenic factors by human tendon cells

21Citations
Citations of this article
38Readers
Mendeley users who have this article in their library.

Abstract

Angiogenesis is associated with the tissue changes underlying chronic overuse tendinopathy. We hypothesized that repetitive, cyclic loading of human tendon cells would lead to increased expression and activity of angiogenic factors. We subjected isolated human tendon cells to overuse tensile loading using an in vitro model (1 Hz, 10% equibiaxial strain). We found that mechanically stimulated human tendon cells released factors that promoted in vitro proliferation and tube formation by human umbilical vein endothelial cells (HUVEC). In response to cyclic strain, there was a transient increase in the expression of several angiogenic genes including ANGPTL4, FGF-2, COX-2, SPHK1, TGF-alpha, VEGF-A and VEGF-C, with no change in anti-angiogenic genes (BAI1, SERPINF1, THBS1 and 2, TIMP1-3). Cyclic strain also resulted in the extracellular release of ANGPTL4 protein by tendon cells. Our study is the first report demonstrating the induction of ANGPTL4 mRNA and release of ANGPTL4 protein in response to cyclic strain. Tenocytes may contribute to the upregulation of angiogenesis during the development of overuse tendinopathy. © 2014 Mousavizadeh et al.

Cite

CITATION STYLE

APA

Mousavizadeh, R., Khosravi, S., Behzad, H., McCormack, R. G., Duronio, V., & Scott, A. (2014). Cyclic strain alters the expression and release of angiogenic factors by human tendon cells. PLoS ONE, 9(5). https://doi.org/10.1371/journal.pone.0097356

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free