Optimization of sample preparation for the identification and quantification of saxitoxin in proficiency test mussel sample using liquid chromatography-tandem mass spectrometry

11Citations
Citations of this article
44Readers
Mendeley users who have this article in their library.

Abstract

Saxitoxin (STX) and some selected paralytic shellfish poisoning (PSP) analogues in mussel samples were identified and quantified with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Sample extraction and purification methods of mussel sample were optimized for LC-MS/MS analysis. The developed method was applied to the analysis of the homogenized mussel samples in the proficiency test (PT) within the EQuATox project (Establishment of Quality Assurance for the Detection of Biological Toxins of Potential Bioterrorism Risk). Ten laboratories from eight countries participated in the STX PT. Identification of PSP toxins in naturally contaminated mussel samples was performed by comparison of product ion spectra and retention times with those of reference standards. The quantitative results were obtained with LC-MS/MS by spiking reference standards in toxic mussel extracts. The results were within the z-score of ±1 when compared to the results measured with the official AOAC (Association of Official Analytical Chemists) method 2005.06, pre-column oxidation high-performance liquid chromatography with fluorescence detection (HPLC-FLD)

Cite

CITATION STYLE

APA

Harju, K., Rapinoja, M. L., Avondet, M. A., Arnold, W., Schär, M., Burrell, S., … Vanninen, P. (2015). Optimization of sample preparation for the identification and quantification of saxitoxin in proficiency test mussel sample using liquid chromatography-tandem mass spectrometry. Toxins, 7(12), 4868–4880. https://doi.org/10.3390/toxins7124853

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free