The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in Drosophila, and in other organisms.
Riabinina, O., Vernon, S. W., Dickson, B. J., & Baines, R. A. (2019). Split-QF system for fine-tuned transgene expression in Drosophila. Genetics, 212(1), 53–63. https://doi.org/10.1534/genetics.119.302034