Split-QF system for fine-tuned transgene expression in Drosophila

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Abstract

The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in Drosophila, and in other organisms.

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Riabinina, O., Vernon, S. W., Dickson, B. J., & Baines, R. A. (2019). Split-QF system for fine-tuned transgene expression in Drosophila. Genetics, 212(1), 53–63. https://doi.org/10.1534/genetics.119.302034

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