Skip to content

Polarizing cytokines for human Th9 cell differentiation

1Citations
Citations of this article
3Readers
Mendeley users who have this article in their library.
Get full text

Abstract

CD4+ T helper (Th) cell subset generation in vivo requires T cell receptor activation and surface CD28 co-stimulation in the presence of one or more cytokines. Similarly, Th cells can be generated in vitro by activating naïve CD4+CD25-T cells with plate bound-anti-CD3 monoclonal antibody (mAb) (pbCD3) and soluble-anti-CD28 mAb (sCD28) in the presence of polarizing recombinant (r) cytokines and anticytokine mAbs. In comparison to in vitro CD4+CD25-T cells, memory CD4+CD25-CD45RO+ T cells have been shown to convert to Th9 cells more efficiently. Here, protocol for in vitro generation of human Th9 cells by activating CD4+CD25−CD45RO+ memory T cells with pbCD3 and sCD28 in the presence of polarizing recombinant interleukin-4 (rIL-4) and transforming growth factor (rTGF-β) is described.

Cite

CITATION STYLE

APA

Putheti, P. (2017). Polarizing cytokines for human Th9 cell differentiation. In Methods in Molecular Biology (Vol. 1585, pp. 73–82). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6877-0_6

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free