Alpha mangostin and xanthone activity on fasting blood glucose, insulin and Langerhans islet of Langerhans in alloxan induced diabetic mice

Abstract

Objective: This research elaborated role of alpha mangostin and xanthone on fasting blood glucose, insulin and langerhans islet in alloxan induced diabetic mice. Methods: Fasting blood glucose, insulin and langerhans islet test were conducted using male Mus musculus mice, divided into 10 groups randomly, which were normal, control (alloxan induced only), glibenclamide, various doses of α-mangostin and xanthone (5, 10, 20 mg/kgbw). Mice were treated for 21 days. Overnight-fasted mice (12 h) were sacrificed by cervical decapitation on day 21 st , following the ethical norms granted by the ethics committee. Fasting blood glucose and insulin plasma were checked. Pancreatic tissues were excised from sacrificed animals, and then fixed in 10 % (v/v) neutral buffered formalin. Histologic observations for Langerhans area were performed after staining using Gomori staining method. Results: The effects of alpha mangostin and xanthone on fasting blood glucose different significantly to control, and were not significantly different from glibenclamide and metformin. Increasing alpha mangostin/xanthone dose from 5 mg/kgbw to 20 mg/bw also did not cause significant differences, although the best results were obtained at a dose of 20 mg/kgbw. Insulin plasma analize showed that there were no significant difference between alpha mangostin/xanthone to normal group, except xanthone 10 mg/kgbw. Langerhans area showed significant difference between alpha mangostin/xanthone to control group. But there's still had significant difference if we compare to glibenclamid/metformin group. Conclusion: Alpha mangostin and xanthone are two substances that showed antidiabetic effect on fasting blood glucose level, insulin plasma and Langerhans islet.