Wheat pathogenesis-related proteins of class 4 have ribonuclease activity

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Abstract

We have demonstrated that wheatwin1, a wheat pathogenesis-related protein of class 4 (PR4), has ribonuclease activity. Both native and recombinant proteins hydrolyse RNA from wheat coleoptils and have antifungal activity. Sepharose-bound wheatwin1 is able to interact with either wheat or Fusarium culmorum RNA. 3D modelling studies showed that, like ribonucleases A and T1, the action mechanism should involve two His residues, an Arg residue and an Asp residue. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

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Caporale, C., Di Berardino, I., Leonardi, L., Bertini, L., Cascone, A., Buonocore, V., & Caruso, C. (2004). Wheat pathogenesis-related proteins of class 4 have ribonuclease activity. FEBS Letters, 575(1–3), 71–76. https://doi.org/10.1016/j.febslet.2004.07.091

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