Novel PDGF aptasensor based on gold nanoparticle triggered chemiluminescence

Abstract

A novel PDGF aptasensor is proposed in this study based on gold nanoparticles (NPs) triggered chemiluminescence (CL) upon target binding. The strategy chiefly depends on the formation of a sandwich-type immunocomplex among the capture antibody immobilized on the 96-well plate, target protein and aptamer-functionalized gold NPs. Introduction of target protein into the assay system leads to the attachment of gold NPs onto the surface of the plates and thus the assembled gold NPs could trigger the reaction between luminol and AgNO 3 with a CL emission. Further signal amplification was achieved by a simple gold metal catalytic deposition onto the gold NPs. This new CL strategy might create a novel technology for developing simple aptasensors in the sensitive and selective detection of target protein in a variety of clinical, environmental and biodefense applications. © 2011 Published by Elsevier Ltd.