In vitro plant development of Eleusine coracana via indirect organogenesis and somatic embryogenesis using mature seeds as explants

Abstract

A complete plant regeneration system has been developed using mature seeds as explants. Mature embryos were used for induction of callus and regeneration of finger millet GE-3885 genotype. Highest callus induction and proliferation was found with MS + 1.5 mg/l NAA and MS+0.5 mg/l NAA respectively. Somatic embryos were obtained in MS + 0.5 mg/l 2,4-D. Histological analysis of somatic embryos revealed globularshaped structure. Plantlets attained good length of shoots and roots on MS + 1.5 mg/l BA and MS + 1.5 mg/l IBA respectively, and were acclimatized under glasshouse conditions after proliferation of roots in hydroponics system.