Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy of these pairs was 4%, which was about expected within experimental error. These results indicate that the nearest-neighbor model is valid for predicting the stability of RNA/DNA hybrid duplexes as well as RNA/RNA and DNA/DNA duplexes. © 1994.
Sugimoto, N., Katoh, M., Nakano, S. ichi, Ohmichi, T., & Sasaki, M. (1994). RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs have identical stability. FEBS Letters, 354(1), 74–78. https://doi.org/10.1016/0014-5793(94)01098-6