Human uterine epithelial RL95-2 and HEC-1A cell-line adhesiveness: the role of plexin B1

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Abstract

Objective: To study the expression of plexin-B1 in high- and low-receptive epithelial-endometrial cell lines, and its possible role in endometrial adhesiveness. Design: Controlled, laboratory study. Setting: Laboratory for Research in Reproductive Sciences, Department of Obstetrics and Gynecology, Ha'Emek Medical Center, Afula, Israel. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): This study was designed to explore and compare the expression and role of plexin-B1 in endometrial cell lines RL95-2 and HEC-1A, used as models of receptive and nonreceptive cells, respectively. The expression of plexin-B1 was analyzed by Western blotting and reverse-transcriptase polymerase chain reaction. The possible role of plexin-B1 in endometrial-trophoblast adhesiveness was studied with attachment and invasion assays. For further validation, we transfected HEC-1A cells with an expressing vector encoded for plexin-B1. Result(s): Significant differences in spheroid attachment was observed between RL95-2 and HEC-1A cells. Western blotting and reverse-transcriptase polymerase chain reaction revealed that in RL95-2 cells, the expression of plexin-B1 was significantly higher. An attachment assay that used RL95-2 cells in the presence of inhibiting antibodies against plexin-B1 significantly decreased the attachment rates of spheroids. A comparison between HEC-1A and transfected HEC-1A (HEC-1A-2) cells showed significant differences in spheroid attachment. No significant difference was found between HEC-1A-2 and RL95-2. An attachment assay using inhibitory antibodies against plexin-B1 significantly decreased the spheroid-attachment rate. Conclusion(s): Based on our results, we think that plexin-B1 contributes to trophoblast-endometrium interactions, most likely by enhancing adhesion properties. © 2007 American Society for Reproductive Medicine.

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APA

Harduf, H., Goldman, S., & Shalev, E. (2007). Human uterine epithelial RL95-2 and HEC-1A cell-line adhesiveness: the role of plexin B1. Fertility and Sterility, 87(6), 1419–1427. https://doi.org/10.1016/j.fertnstert.2006.11.141

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