Six multiplex-compatible PCR primers were designed to distinguish Streptococcus pneumoniae serotypes within serogroup 18 from culturable/nonculturable pneumococcal specimens, with no cross-reactivity with other serotypes and respiratory organisms. These primers will aid in the generation of better data on vaccine/nonvaccine serotypes in invasive and carriage pneumococcal surveillance and contribute to future vaccine formulation and impact studies.
Tanmoy, A. M., Saha, S., Darmstadt, G. L., Whitney, C. G., & Saha, S. K. (2016). PCR-based serotyping of streptococcus pneumoniae from culture-negative specimens: Novel primers for detection of serotypes within serogroup 18. Journal of Clinical Microbiology, 54(8), 2178–2181. https://doi.org/10.1128/JCM.00419-16