Biosynthesis of Alpha Amylase by Chemically Treated Mutant of Bacillus subtilis

Abstract

The present study is concerned with the selection of hyper-producer strain of Bacillus subtilis and optimization of the cultural conditions for the production of alpha amylase in shake flask. The UV irradiated strain of Bacillus subtilis was treated with NTG for different intervals of time (5-60 min). One hundred mutant strains were isolated and tested for the production of alpha amylase and Bacillus subtilis GCBCM-25 gave maximum production of enzyme (2210 U/ml). The optimum conditions for the production of alpha amylase were, NaNO3 as nitrogen source, pH 7.5, phosphate buffer as diluent and 4mM CaCl2. The treatment of NTG for 25 min was found to be suitable for the production of best mutant of Bacillus. The production of alpha amylase was significantly increased with the addition of NaNO3 and 4 mM CaCl2 to the fermentation medium.