Biochar addition increased soil bacterial diversity and richness: Large-scale evidence of field experiments

Abstract

Biochar plays a crucial role in enhancing soil ecological functions and productivity, and mitigating environmental pollution. Despite the available studies conducted through high-throughput sequencing to understand the effects of biochar on soil bacterial diversity and richness, a comprehensive understanding remains elusive. Our global meta-analysis addresses this knowledge gap, incorporating 473 pairs of observations from 84 studies to assess soil bacterial diversity and richness response to biochar addition. We found that biochar application increased bacterial Shannon and Chao1 indices by 0.99 % and 6.45 % respectively. However, these positive effects were context-dependent, especially concerning bacterial diversity. Through aggregated boosted trees analysis, we determined that soil characteristics (including soil organic carbon and nitrogen contents, soil pH, and soil texture) had a more significant influence than biochar properties, experimental conditions, or climatic factors on soil bacterial diversity and richness post biochar addition. In particular, the soil carbon to nitrogen ratio stood out as the leading factor influencing the bacterial Shannon and Chao1 indices following biochar addition. Our findings offer new insights into biochar's influence on soil bacterial activity, taking into account biochar-mediated spatiotemporal variation. This information is pivotal for optimizing biochar characteristics and application to improve soil biological health.