Perspectives for the microbial production of methyl propionate integrated with product recovery

7Citations
Citations of this article
26Readers
Mendeley users who have this article in their library.

Abstract

A new approach was studied for bio-based production of methyl propionate, a precursor of methyl methacrylate. Recombinant E. coli cells were used to perform a cascade reaction in which 2-butanol is reduced to butanone using alcohol dehydrogenase, and butanone is oxidized to methyl propionate and ethyl acetate using a Baeyer-Villiger monooxygenase (BVMO). Product was removed by in situ stripping. The conversion was in line with a model comprising product formation and stripping kinetics. The maximum conversion rates were 1.14 g-butanone/(L h), 0.11 g-ethyl acetate/(L h), and 0.09 g-methyl propionate/(L h). The enzyme regioselectivity towards methyl propionate was 43% of total ester. Starting from biomass-based production of 2-butanol, full-scale ester production with conventional product purification was calculated to be competitive with petrochemical production if the monooxygenase activity and regioselectivity are enhanced, and the costs of bio-based 2-butanol are minimized.

Cite

CITATION STYLE

APA

Pereira, J. P. C., van der Wielen, L. A. M., & Straathof, A. J. J. (2018). Perspectives for the microbial production of methyl propionate integrated with product recovery. Bioresource Technology, 256, 187–194. https://doi.org/10.1016/j.biortech.2018.01.118

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free