Identification of Gene Positioning Factors Using High-Throughput Imaging Mapping

87Citations
Citations of this article
323Readers
Mendeley users who have this article in their library.

Abstract

Summary Genomes are arranged non-randomly in the 3D space of the cell nucleus. Here, we have developed HIPMap, a high-precision, high-throughput, automated fluorescent in situ hybridization imaging pipeline, for mapping of the spatial location of genome regions at large scale. High-throughput imaging position mapping (HIPMap) enabled an unbiased siRNA screen for factors involved in genome organization in human cells. We identify 50 cellular factors required for proper positioning of a set of functionally diverse genomic loci. Positioning factors include chromatin remodelers, histone modifiers, and nuclear envelope and pore proteins. Components of the replication and post-replication chromatin re-assembly machinery are prominently represented among positioning factors, and timely progression of cells through replication, but not mitosis, is required for correct gene positioning. Our results establish a method for the large-scale mapping of genome locations and have led to the identification of a compendium of cellular factors involved in spatial genome organization.

Cite

CITATION STYLE

APA

Shachar, S., Voss, T. C., Pegoraro, G., Sciascia, N., & Misteli, T. (2015). Identification of Gene Positioning Factors Using High-Throughput Imaging Mapping. Cell, 162(4), 911–923. https://doi.org/10.1016/j.cell.2015.07.035

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free