Calumenin interacts with serum amyloid P component

38Citations
Citations of this article
30Readers
Mendeley users who have this article in their library.

Abstract

We recently reported the identification of human calumenin, a novel Ca2+ binding, transformation-sensitive and secreted protein [Vorum et al. (1998) Biochim. Biophys. Acta 1386, 121-131; Vorum et al. (1999) Exp. Cell Res. 248, 473-481] belonging to the family of multiple EF-hand proteins of the secretory pathway that include reticulocalbin, ERC-55, Cab45 and crocalbin. In order to further investigate the extracellular functions of calumenin we immobilized the recombinant protein to a column. After application of a placental tissue extract we were able to elute one protein that interacts with calumenin in the presence of Ca2+. Amino acid sequencing identified this protein as serum amyloid P component (SAP). Furthermore, we verified and characterized the calumenin-SAP interaction by the surface plasmon resonance technique. The findings indicate that calumenin may participate in the immunological defense system and could be involved in the pathological process of amyloidosis that leads to formation of amyloid deposits seen in different types of tissues. © 2000 Federation of European Biochemical Societies.

Cite

CITATION STYLE

APA

Vorum, H., Jacobsen, C., & Honoré, B. (2000). Calumenin interacts with serum amyloid P component. FEBS Letters, 465(2–3), 129–134. https://doi.org/10.1016/S0014-5793(99)01734-2

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free