An in vitro analysis of surface characterization of titanium discs with novel polyelectrolytic hydrogel for implants tissue engineering

Abstract

Background: Conventional implant surfaces is being challenged leading to development of various additive and subtractive surface modifications promoting increased and faster bone to implant contact. In this research Cissus quadrangularis (CQ), hyloronic acid (HA) and chitosan (CH)composite polyelectrolyte solution was prepared for surface treatment of titanium implants. Aim/Hypothesis: We have hypothesized that the next‐generation scaffold will be a biologically active, antimicrobial and functionally in liquid form closely mimic the native ECM. Aim‐ To develop bioactive antimicrobial scaffold for the expression of enhanced bone tissue engineering Material and Methods: (i) CH was dissolved in diluted acetic acid and a pH was adjusted to neutral using 0.1 N NAOH, HA was added to CH. (ii) CQ Extract was prepared by maceration technique, The extract obtained was incorporated into CH. (iii) (CH‐ HA‐ CQ) was prepared by mixing both the above mentioned in 50–50 by weight. IN VITRO ANALYSIS. TheCH CQ HA solution was coated onto titanium discs and was kept at temperature of 37 0 and was then transferred to vitro culture analysis. The hydrogel was characterized by FT‐IR spectrometry b. CELL VIABILITY ASSAY ‐The proliferation assay was assessed by MTT assay. Fibroblast cells were seeded onto the CH CQ HA coated discs and cell proliferation was monitored after 72 hours of incubation. samples was subjected to spectrophotometer at 570 nm. ALP analysis ‐Mg‐63 cells were seeded onto the disc surfaces and cultures were cultivated for 14 days. The density of _I_p_i_‐nitrophenol was determined. Results: The antibacterial activity of CH CQ HA showed effective results against bacteria. MTT assay revealed significant changes in proliferation of osteoblast cells (0.24 0.007) compared with control (0.22 0.007) (_I_P_i_ < 0.34). While Alkaline phosphatase activity of MG 63 osteoblast like cells growing in the presence of a (CQ CH HA) composite had the highest value of the ALP activity (0.23 0.005) compared with control (0.22 0.005) (_I_P_i_ < 1). Conclusions and Clinical Implications: The present work demonstrates an polyelectrolytic scaffold which is biomimetic, angiogenic, and antimicrobial with considerable potential as excellent scaffolds for cellular adhesion, proliferation, and differentiation. Finally, as a future outlook, the use of a hydro gel as a scaffold around the implant is highlighted as a promising approach for implant tissue engineering [ABSTRACT FROM AUTHOR]