The measurement of nitrite in adulterated urine samples by high- performance ion chromatography

Abstract

With the increased availability of nitrite-containing commercial products that are used for the adulteration of urine samples in the workplace, it is necessary for laboratories to be able to detect and confirm the presence of nitrite in these samples. We have developed a method to confirm the presence of nitrite in urine samples. The method uses the IonPac AS 14 analytical column with the Dionex series 45001 Bio LC system equipped with an anion self-generating suppressor and conductivity detector. Using a single-point calibration, the method is linear and accurately quantitates nitrite to 12,000 μg/mL. The limit of detection is 30 μg/mL, and the day- to-day precision of the assay has a coefficient of variation (CV) of 4.3% at 1200 μg/mL and 3.8% at 2700 μg/mL of nitrite.