Investigated of the Hydrogen peroxide's efficacy in sterilizing various substrates for the formation of the oyster mushroom Pleurotus sapidus

Abstract

This study was conducted in order to test some agricultural wastes from fruits and sterilization method in the production of oyster mushroom Pleurotus sapidus. This study included two factors, the first is the type of substrate which consisting of seven combinations: A0 (wheat straw 80% + flour bran 20%), A1 (pomegranate peel 25% + wheat straw 75%), A2 (pomegranate peel 50% + wheat straw 50%), A3 (pomegranate peel 75% + wheat straw 25%), A4 (date fruit residues 25% + wheat straw 75%), A5 (date fruit residues 50% + wheat straw 50%), A6 (date fruit residues 75% + straw wheat 25%). The second factor is the sterilization method, represented by two methods of sterilization, the first is sterilization by Autoclave (P0) and the second is sterilization using hydrogen peroxide H2O2 at two concentrations of 3% (P1) and 5% (P2). A2 achieved the shortest period of growth and spread of mycelium which was 51.67 days and the highest amount of total wet yield was 199.2 g.kg-1. A0 and A6 had the shortest period for primordia formation which was 61.0 and 62.8 days, respectively. The mixture of A6 substrate gave the longest production cycle duration of 40.8 days and the highest biological efficiency rate was 44.78%. A1 and A2 gave the shortest fruiting period of 6.22 days. The P0 sterilization method recorded the shortest period for the growth and spread of mycelium, shortest period for the formation of primordia and the longest production cycle resulting in 48.88, 47.4 and 43.6 days, respectively, while the P1 sterilization method recorded highest wet yield of 175.6 g.kg-1 and the highest biological efficiency rate of 39.85%. Copyright: