Chromatographic fractionation of rat tail tendon collagen

Abstract

PHYSICAL studies1 indicate that solubilized collagens give rise to two components when heated gently (13°-40° C. depending on the source of the collagen)2 or when treated with other agents which break weak bonds. These two components have not been satisfactorily isolated3 by the methods used to fractionate4 'gelatine', an end-product of more drastically altered collagen. We have succeeded in chromatographing warm dissolved rat tail tendon collagen into four components with a method which allows for the isolation of these components. © 1959 Nature Publishing Group.