Spectrophotometric determination of atenolol via oxidation and bleaching color reaction for methyl red dye

Abstract

A selective and sensitive spectrophotometric method has been suggested for the quantitative assay of atenolol (ATNL) as pure and in its manufactural formulation(Tablet). The suggested procedure included oxidation of ATNL with an excess quantity of the oxidant N-bromosuccinimide (NBS), and then the excess of NBS was occupied in bleaching the color of methyl red dye(MRD), then measuring the absorbance of remaining MRD at 518 nm . The absorbance of the unbleached color of MRD corresponds to the ATNL concentration in the sample solution. Beer's law was followed in the range of 0.1-2.0 μg.ml-1with molar absorptivity value equal to 8.8864x104 l.mol-1 .cm-1. The suggested method was applied to the assay of ATNL in commercial tablets, with satisfactory results.