Endothelial function after prolonged coronary artery oxygen persufflation in a rabbit model of heart preservation

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Abstract

Coronary oxygen persufflation may serve as a means to improve storage conditions and organ preservation time for cardiac transplantation. We examined whether coronary oxygen persufflation and prolonged preservation time alter the endothelium-dependent relaxation of isolated coronary arteries. Isolated rabbit hearts were subjected to four different protocols: control (no preservation), 3 h cold storage in Bretschneider's solution, 18 h cold storage in Bretschneider's or University of Wisconsin solution, combined with coronary oxygen persufflation. After 2 h parabiotic reperfusion, intramural segments of coronary arteries were isolated and isometric tension was recorded using a small-vessel myograph. Endothelial function was examined using carbachol and substance P, applied after vessel constriction using high (30 mmol/l) K + or U 46.619, a thromboxane receptor agonist. In another series, coronary flow was measured after Bretschneider's ±18 h coronary oxygen persufflation, or in freshly isolated, retrogradely perfused Langendorff hearts. Flow responses to substance P, acetylcholine or bradykinin were recorded. In saline-reperfused intact hearts no change in the normal effects of endothelium-dependent relaxants was detected after 18 h, irrespective of coronary oxygen persufflation. However, after isolation of the resistance vessels endothelium-dependent relaxation was abolished after long-term preservation and persufflation. Similar results were obtained after mechanical removal of the endothelium using control hearts. Short-term preservation without persufflation resulted in relaxations similar to those in non-preserved control hearts. Long-term preservation of rabbit heart including coronary oxygen persufflation results in unchanged endothelium-dependent relaxation in intact heart, but abolishes the endothelium-dependent relaxation after isolation of the vessels. © 2002 Elsevier Science B.V. All rights reserved.

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Kunze, S., Jeschkeit-Schubbert, S., Dahnken, S., Fischer, J. H., & Herzig, S. (2002). Endothelial function after prolonged coronary artery oxygen persufflation in a rabbit model of heart preservation. Interactive Cardiovascular and Thoracic Surgery, 1(1), 16–22. https://doi.org/10.1016/S1569-9293(02)00004-X

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