Expression of hENTl and ERCC1 genes in tumor tissues non-small cell lung cancer

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Abstract

Objective: To investigate the expression of hENTl and ERCC1 genes in tumor tissues non-small cell lung cancer (NSCLC). Methods: Fresh non-small lung cancer specimens were transplanted into nude mice. Twenty mice were randomized into two groups: experimental group receiving gemcitabine plus cisplatin and control group receiving 0.9% physiological saline. The expressions of hENTl and ERCC1 mRNA in tumor tissue were detected by real-time fluorescent quantitative PCR. The volume of tumor, the weight of nude mice and tumor volume were respectively measured and calculated 2-3 times per week. Tissue samples were collected from NSCLC mice treated with gemcitabine plus carboplatin. Results: The histological examination showed that many tumor cells were well preserved in nude mice. The rate of transplanted tumor cells was 86.7%. The concomitant treatment study showed that the rate of TV, RTV, T/C in GEM + DDP group was the lowest. LBP + DOC, DDP + DOC obviously influenced the body weight. Compared with NS group, DDP group, GEM group, the survival period and the level of hENTl of DDP+GEM group increased obviously, the level of ERCC1 decreased significantly (P<0.05). Conclusions: The expression of hENT1 and ERCC1 genes in tumor tissues were closely correlated with the response to chemotherapy and prognosis of patients with NSCLC treated with gemcitabine plus cisplatin. © 2013 Hainan Medical College.

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Wu, J. J., & Jiao, S. C. (2013). Expression of hENTl and ERCC1 genes in tumor tissues non-small cell lung cancer. Asian Pacific Journal of Tropical Medicine, 6(11), 908–911. https://doi.org/10.1016/S1995-7645(13)60162-5

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