There is a general feeling that the routinely measured semen parameters (sperm number, motility, vitality, morphology) do not monitor sperm function and that they are inadequate for predicting fertility (Lewis 2007). Whereas routine morphological staining may be complemented by specifi c lectin or immunocytochemical stains of particular sperm structures (WHO 1999a, b; Gomez et al. 1996), additional investigations, in which certain functions of the spermatozoon are assessed, may provide clinically useful prognostic or diagnostic information. These tests should distinguish between fertile and infertile men or indicate the reason for the infertility and hence be of use in suggesting a rational therapy. This chapter discusses the different cellular aspects required of spermatozoa during fertilization that can be tested.
Yeung, C. H., & Cooper, T. G. (2010). Sperm quality and function tests. In Andrology: Male Reproductive Health and Dysfunction (pp. 139–154). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-540-78355-8_10