Identification of Gremmeniella abietina races with random amplified polymorphic DNA markers

Abstract

Seven random amplified polymorphic DNA (RAPD) markers amplified from four oligonucleotides (10-mers) by the polymerase chain reaction were used to distinguish between the North American and European races of Gremmeniella abietina, the causal agent of Scleroderris canker of conifers. Forty-three isolates of the pathogen from 11 different host species originating from 11 countries, states, and provinces were tested; race designation was consistent with results from immunogenic and soluble-protein assays. By using RAPD markers, it was possible to identify G. abietina races by DNA amplifications directly from fruiting bodies, thus eliminating the need to culture the fungus, as is necessary with immunogenic and soluble-protein assays. Two isolates which had been previously classified as intermediate were clearly identified as belonging to either one of the two races by using RAPD markers. No interracial hybrids were detected in our survey. Patterns of amplification products from the European race in North America were identical to patterns of European isolates, further substantiating that this is an introduced race to the North American continent.