Flavonoids from the Leaves of Bridelia stipularis with in Vitro Antioxidant and Cytotoxicity Activity

Abstract

Methanolic extract of the leaves of Bridelia stipularis was studied. From this study, we isolated three known flavonoids. They were identified as 7-O-methyl luteolin, apigenin and 5, 7, 2', 5' tetrahydroxyflavone by NMR spectroscopic studies. All of them are first time documented for this plant. Different solvent fractions were subjected to in vitro antioxidant and cytotoxicity studies. Both apigenin and ethyl acetate soluble fraction of Bridelia stipularis showed strong antioxidant activity having IC 50 value of 8.005, 8.77 µg/mL respectively. Chloroform soluble fraction of Bridelia stipularis exerted the highest toxicity to brine shrimp and petroleum ether soluble fraction showed moderate toxicity having LC 50 value of 1.05, 1.71 µg/mL respectively.