Laboratory techniques for human embryos

22Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.

Abstract

This review is concerned with laboratory techniques needed for assisted conception, particularly the handling of gametes and embryos. Such methods are being increasingly refined. Successive stages of fertilization and embryogenesisrequire especial care, and often involve the use of micromanipulative methods for intracytoplasmic sperm injection (ICSI) or preimplantation genetic diagnosis. Embryologists must take responsibility for gamete collection and preparation, and for deciding on the means of insemination or ICSI. Embryos must be assessed in culture, during the 1-cell, cleaving and morula/blastocyst stages, and classified according to quality. Co-culture methods may be necessary. The best embryos for transfer must be selected and loaded into the transfer catheter. Embryos not transferred must be cryopreserved, whichdemands the correct application of current methods of media preparation, seeding and the correct speed for cooling and warming. Before too long, methods of detecting abnormal embryos and avoiding their transfer may become widespread. ©2002 Elsevier Science Ltd. All rights reserved.

Cite

CITATION STYLE

APA

Geber, S., Sales, L., & Sampaio, M. A. C. (2002). Laboratory techniques for human embryos. Reproductive Biomedicine Online. https://doi.org/10.1016/S1472-6483(10)61625-2

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free