DETERMINATION OF ETHYLENEDIAMINETETRAACETIC ACID AND NITRILOTRIACETIC ACID AS THEIR IRON(III)-COMPLEXES BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY.

Abstract

The iron(III) complexes with ethylenediaminetetraacetic acid (EDTA, H//4edta) and nitrilotriacetic acid (NTA, H//3nta) were separated by the ion-association reversed phase high performance liquid chromatography. The retention behavior of iron(III)-nta complex on the column (4. 6 mm i. d. , 25 cm long) packed with tetrabutylammonium bromide and acetonitrile. A mixture of edta and nta in a synthetic sample solution containing the commonly present metal ions was converted to the corresponding iron(III) complexes and determined successfully by the present method.