Objective: To evaluate the GH-releasing hormone (GH-RH)-induced response of GH in patients affected by hypothalamic amenorrhea. Design: Patients affected by weight-loss-related hypothalamic amenorrhea (n = 28) were studied and compared with 20 healthy controls. Among patients with weight-loss amenorrhea, both hypogonadotropic and normogonadotropic conditions were present. All subjects underwent a GH-RH test (GEREF, Serono, Rome, Italy) (1 μg/kg body weight IV). Plasma GH concentrations were determined using commercially available RIAs. Also, in selected samples insulin-like growth factor-I (IGF-I) levels were measured. Results: Basal plasma IGF-I levels as well as body mass index (BMI) were lower in amenorrheic patients than in healthy controls. No significant correlation was found between BMI and IGF-I or E2 plasma levels or between LH and IGF-I plasma levels. The basal GH plasma levels were comparable in all groups of subjects. The GH-RH-induced GH response evaluated as maximal release and as area under the curve (AUC) was higher in amenorrheic patients than in control subjects. Conclusions: The amenorrheic condition associated with reduced BMI changes the GH-RH-induced GH response in hypothalamic amenorrhea, supporting a GH and a IGF-I disregulation in weight-loss-related amenorrhea.
Genazzani, A. D., Petraglia, F., Gastaldi, M., Gamba, O., Corazza, F., D’Ambrogio, G., & Genazzani, A. R. (1996). Growth hormone (GH)-releasing hormone-induced GH response in hypothalamic amenorrhea: Evidence of altered central neuromodulationa. Fertility and Sterility, 65(5), 935–938. https://doi.org/10.1016/S0015-0282(16)58264-8