The structure of cerexin B. Studies on antibiotics from the genus bacillus. XVII

Abstract

The structure of cerexin B was examined. The constituent fatty acids were elucidated by gas chromatography and mass spectrometry to be β-hydroxy isodecanoic acid, β-hydroxy decanoic acid, β-hydroxy isoundecanoic acid and β-hydroxy anteisoundecanoic acid. The configurations of constituent amino acids were determined as asparagine (2D, 1L), valine (D), phenylalanine (D), allo-threonine (D), tryptophan (D), and allo-isoleucine (D) from their optical activities. Treatment with conc. hydrochloric acid cleaved at the γ-hydroxylysine residue to give two peptide fragments, one of which (the N-terminal side) was then deacylated with Polymyxin Acylase. Their amino acid sequences were examined by EDMAN degradation. From the results and analogy to cerexin A, the structure of cerexin B was deduced. © 1976, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.