Multiple initiation mechanisms adapt phage T4 DNA replication to physiological changes during T4's development

49Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

We summarize the evidence for multiple pathways to initiate phage T4 DNA replication. In any infecting chromosome, leading DNA strands can be primed from pre-replicative transcripts, independent of primase activity, at one of several origins. Within each origin region, there are multiple RNA-DNA transition sites. However, the priming potential at each single site is very low. Our results suggest that origin transcripts can become primers for leading strand DNA synthesis without being processed, but that a promoter-proximal segment of each origin transcript plays an important structural role, as a proposed wedge, in the transition from RNA to DNA synthesis. Two recombination-dependent pathways render subsequent phage T4 DNA replication independent of transcription. The first of these requires proteins that are synthesized during the pre-replicative phase of infection. It is active as soon as the first growing points, initiated at origins, have reached a chromosomal end. The other one requires at least one late protein: endonuclease VII, a resolvase that cuts recombinational junctions. The latter pathway can bypass primase deficiencies by allowing retrograde DNA synthesis without Okazaki pieces. We discuss the integration of these multiple and redundant pathways into the developmental program of T4. Competition between these initiation mechanisms and with other DNA transactions allows for integration of replication controls with transcription, recombination and packaging of the DNA. © 1995.

Cite

CITATION STYLE

APA

Mosig, G., Colowick, N., Gruidl, M. E., Chang, A., & Harvey, A. J. (1995). Multiple initiation mechanisms adapt phage T4 DNA replication to physiological changes during T4’s development. FEMS Microbiology Reviews, 17(1–2), 83–98. https://doi.org/10.1016/0168-6445(94)00069-7

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free