The use of soil biofilms for observing protozoan movement and feeding

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Abstract

Protozoan movement and feeding regimes in soil biofilms were observed with inverted microscopes and Utermöhl plankton counting chambers (4 mm deep). In a new use for these counting chambers, the three-dimensional appearance of a soil pore network can be simulated using long working distance objectives (40×). Protozoa were often associated with soil surfaces and penetrated soil crevices of less than 3 μm in diameter by either distorting their body outlines or with slender pseudopodia of less than 2 μm at the tips. Many ciliates repeatedly collided with soil particles and released attached bacteria into the soil solution for subsequent predation. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

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Darbyshire, J. F. (2005). The use of soil biofilms for observing protozoan movement and feeding. FEMS Microbiology Letters, 244(2), 329–333. https://doi.org/10.1016/j.femsle.2005.02.001

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