cDNAs complementary to pig kidney mRNAs coding for α- and β-subunits of Na+,K+-ATPase were cloned and sequenced. Selective tryptic hydrolysis of the α-subunit within the membrane-bound enzyme and tryptic hydrolysis of the immobilized isolated β-subunit were also performed. The mature α- and β-subunits contain 1016 and 302 amino acid residues, respectively. Structural data on the peptides from extramembrane regions of the α-subunit and on glycopeptides of the β-subunit underlie a model for the transmembrane arrangement of Na+,K+-ATPase polypeptide chains. (Na+ + K+)-ATPase α-Subunit β-Subunit cDNA nucleotide sequence Primary structure Glycopeptide Transmembrane arrangement. © 1986.
Ovchinnikov, Y. A., Modyanov, N. N., Broude, N. E., Petrukhin, K. E., Grishin, A. V., Arzamazova, N. M., … Sverdlov, E. D. (1986). Pig kidney Na+,K+-ATPase. Primary structure and spatial organization. FEBS Letters, 201(2), 237–245. https://doi.org/10.1016/0014-5793(86)80616-0