Lipid peroxidation in mitochondria induced by Fe2+ in the presence of ascorbate or by cumene hydroperoxide in the presence of phosphate results in a drop of the membrane potential and in K+ efflux. The inhibitors of ATP-synthetase (oligomycin and dicyclohexylcarbodiimide (DCCD)) are capable of preventing lipid peroxidation, stabilizing the membrane potential and inhibiting potassium efflux. The same effects are observed in the presence of ionol or α-tocopherol. In contrast to antioxidant protection the effects of oligomycin and DCCD are reversed by the uncoupler (FCCP). The functional link between non-enzymatic lipid peroxidation, proton conduction through Fo component of ATP-synthetase and induced cation transport is suggested. © 1983.
Marshansky, V. N., Novgorodov, S. A., & Yaguzhinsky, L. S. (1983). The role of lipid peroxidation in the induction of cation transport in rat liver mitochondria. The antioxidant effect of oligomycin and dicyclohexylcarbodiimide. FEBS Letters, 158(1), 27–30. https://doi.org/10.1016/0014-5793(83)80669-3