The sweet and musky scent of home: biogenic ethylene fine-tunes seed germination in wetlands

Abstract

Wetlands are known for comparatively high production of biogenic ethylene from decomposed sediment. Because the gas has various well-documented effects on seed physiology, we asked whether it can be a vital seed germination cue for wetland plants. Specifically, we explored whether ethylene plays an ecological role in (i) breaking/weakening seed dormancy, (ii) broadening the germination niche width, (iii) promoting germination speed or (iv) altering the germination requirements of six plant species with different occurrence along a hydroperiod gradient. In a factorial experiment, both ethylene-treated and untreated seeds were incubated in combinations of temperature (constant versus fluctuating), illumination (light versus darkness) and oxygen (aerobic versus hypoxia) with and without cold stratification. Our results revealed seed exposure to ethylene did not weaken or break dormancy without cold stratification treatment. However, ethylene helped to broaden the germination niche width, increased overall germination percentage and speed of cold-stratified (non-dormant) seeds. This indicates that ethylene helps those seeds that lost dormancy (non-dormant) to sense favourable water-saturated versus flooded substrate depending on their requirement for aerobic versus hypoxic conditions to trigger germination. We conclude that ethylene does not interfere directly with the dormancy-breaking process in autumn-dispersed seeds that are naturally cold-stratified in winter and germinate in spring/summer. However, ethylene plays a crucial ecological role as a ‘flood detector' for different wetland plant communities (reed, mudflat, swamp, shallow water) to synchronize germination of non-dormant seeds in the most suitable habitat at the right time.