Defining the active site of Schizosaccharomyces pombe C-terminal domain phosphatase Fcp1

Abstract

Fcp1 is an essential protein serine phosphatase that dephosphorylates the C-terminal domain (CTD) of RNA polymerase II. By testing the effects of serial N- and C-terminal deletions of the 723-amino acid Schizosaccharomyces pombe Fcp1, we defined a minimal phosphatase domain spanning amino acids 156-580. We employed site-directed mutagenesis (introducing 24 mutations at 14 conserved positions) to locate candidate catalytic residues. We found that alanine substitutions for Arg223, Asp258, Lys280, Asp297, and Asp298 abrogated the phosphatase activity with either p-nitrophenyl phosphate or CTD-PO4 as substrates. Structure-activity relationships were determined by introducing conservative substitutions at each essential position. Our results, together with previous mutational studies, highlight a constellation of seven amino acids (Asp170, Asp172, Arg223, Asp258, Lys280, Asp297, and Asp298) that are conserved in all Fcp1 orthologs and likely comprise the active site. Five of these residues (Asp170, Asp172, Lys280, Asp297, and Asp298) are conserved at the active site of T4 polynucleotide 3′-phosphatase, suggesting that Fcp1 and T4 phosphatase are structurally and mechanistically related members of the DXD phosphotransferase superfamily.