Effects of calcium signaling on coagulation factor VIIa-induced proliferation and migration of the SW620 colon cancer cell line

Abstract

Tissue factor (TF)/VIIa/protease-activated receptor 2 (PAR2) has been shown to trigger the ERK1/2 signaling pathway. This was shown to be closely associated with the proliferation and migration of SW620 colon cancer cells; however, the detailed mechanisms remain unclear. The aim of the present study was to elucidate the effects of calcium signaling on the proliferation and migration of SW620 cells induced by coagulation factor VIIa. The results demonstrated that VIIa and PAR2 agonist PAR2-AP increased [Ca2+]i in SW620 cells. In addition, VIIa-and PAR2-AP-induced ERK1/2 activation was inhibited by thapsigargin (TG)-induced depletion of intracellular Ca2+ stores and EGTA-mediated removal of extracellular Ca2+. It was also identified that VIIa and PAR2-AP-induced proliferation and migration of SW620 cells was modulated by EGTA and TG. Taken together, the present results indicate that VIIa triggers calcium signaling in SW620 cells, in a TF-dependent manner, which is critical for VIIa-induced ERK1/2 activation in SW620 cells. These results suggested that calcium signaling had a vital role in the proliferation and migration of SW620 cells.