Interrogating the repertoire: Broadening the scope of peptide-MHC multimer analysis

Abstract

Labelling antigen-specific T cells with peptideĝ€"MHC multimers has provided an invaluable way to monitor T cell-mediated immune responses. A number of recent developments in this technology have made these multimers much easier to make and use in large numbers. Furthermore, enrichment techniques have provided a greatly increased sensitivity that allows the analysis of the naive T cell repertoire directly. Thus, we can expect a flood of new information to emerge in the coming years. © 2011 Macmillan Publishers Limited. All rights reserved.