Endemic Burkitt lymphoma (eBL) is the most common childhood cancer in equatorial Africa and is linked to Epstein-Barr virus (EBV) and Plasmodium falciparum coinfections early in life. Epstein-Barr nuclear antigen 1 (EBNA1) is the sole viral latent antigen expressed in BL tumors. Loss of EBNAl-specific immune surveillance could allow eBL emergence. Therefore, EBNA1-specific T cell responses were analyzed by IFN-y ELISPOT in Kenyan children with eBL and compared to healthy children with divergent malaria exposure. Significantly fewer children with eBL, 16% (7/44) had EBNAl-specific IFN-y responses in contrast to healthy children living in a malaria holoendemic area or in an area with sporadic malaria transmission, 67% (40/60) and 72% (43/60) responders, respectively (p < 0.003). Children with eBL maintained IgG1 dominated antibody responses to EBNA1 similar to healthy children suggesting a selective loss of IFN-c secreting EBNA1-specific T cells in the presence of intact humoral immunity. CD8 + T cell responses to EBV lytic and latent antigens not expressed in the tumors were similarly robust in eBL patients compared to healthy children. In addition, CD4 + T cell responses to a malaria protein, merozoite surface protein 1, were present in lymphoma patients. This study demonstrates a selective loss of EBNA1-specific T cell responses in children with eBL and suggests a potential immunotherapeutic target for this EBV-associated lymphoma. © 2008 Wiley-Liss, Inc.
CITATION STYLE
Moormann, A. M., Heller, K. N., Chelimo, K., Embury, P., Ploutz-Snyder, R., Otieno, J. A., … Rochford, R. (2009). Children with endemic Burkitt lymphoma are deficient in EBNAl-specific IFN-γ T cell responses. International Journal of Cancer, 124(7), 1721–1726. https://doi.org/10.1002/ijc.24014
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