Preparation, Characterization and Evaluation of Prasugrel Hydrochloride Nanosuspensions: Its enhancement of dissolution rate and oral bioavailability

Abstract

The objective of this study was to investigate nanosuspensions for enhancing the solubility and dissolution rate of Prasugrel HCl (PHCl) so as to reduce the fluctuations in its oral bioavailability. Prasugrel Hydrochloride nanosuspensions were prepared using evaporative precipitation method. After preparation, various nanosuspensions were characterized for particle size, scanning electron microscope (SEM), zeta potential, drug entrapment efficiency (EE), Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). Solubility and in-vitro release of the drug from nanoparticles are determined. The formulations were characterized using techniques such as powder x-ray diffractometry, scanning electron microscopy, in-vitro dissolution and in-vivo absorption in rats. The dissolution rate and oral absorption of Prasugrel Hydrochloride in the form of nanosuspensions was significantly higher than that of oral suspension and pure drug. All the techniques investigated in this study can be used to enhance dissolution rate and oral absorption of prasugrel hydrochloride and thus can reduce the fluctuations in its oral bioavailability. Nanosuspensions demonstrated to be better and superior technique when compared to other techniques investigated in enhancing oral bioavailability of Prasugrel Hydrochloride. Prasugrel hydrochloride nanosuspension was successfully developed using and the bioavailability is 4 folds increased than its pure drug.