Increasing fidelity and efficiency by modifying cytidine base-editing systems in rice

1Citations
Citations of this article
4Readers
Mendeley users who have this article in their library.

Abstract

The efficiency of plant cytidine base-editing systems is limited, and unwanted mutations frequently occur in transgenic plants. We increased the cytidine editing frequency and fidelity of the plant base editor 3 (BE3) and targeted activation-induced cytidine deaminase (CDA) (target-AID) systems by coexpressing three copies of free uracil–DNA glycosylase (UDG) inhibitor (UGI). The editing efficiency of the improved BE3 and CDA systems reached as high as 88.9% and 85.7%, respectively, in regenerated rice plants, with a very low frequency of unwanted mutations. The low editing frequency of the BE3 system in the GC context could be overcome by the modified CDA system. These results provide a high-fidelity and high-efficiency solution for rice genomic base editing.

Cite

CITATION STYLE

APA

Qin, R., Liao, S., Li, J., Li, H., Liu, X., Yang, J., & Wei, P. (2019). Increasing fidelity and efficiency by modifying cytidine base-editing systems in rice. Crop Journal. https://doi.org/10.1016/j.cj.2019.04.007

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free