Transferable polymorphic microsatellite markers from Capsicum annuum to Capsicum baccatum

Abstract

Several researches have extensively used microsatellite markers for genetic mapping, because of their high transferring values among species from the same genus. This work aims at evaluating transferability of microsatellite locus developed to Capsicum annuum in a mapping population of Capsicum baccatum. We have tested 152 pairs of microsatellite primers developed to C. annuum in a mapping population constituted by 203 individuals F2 originated from crossing between two accessions of C. baccatum var. pendulum, accomplishing a previous optimization of primers annealing temperatures. On the map construction, we have employed only the polymorphic markers and that generated clear marks, totalizing 62 markers transferred with success, 42 mapped, resulting in a percentage of 40.8% transferred microsatellites. Furthermore, we observed a relevant correspondence between microsatellite markers allocation on linkage groups C. baccatum and linkage groups C. annuum. This is the first report on the transfer of microsatellite markers to a species of C. baccatum, being an important advance on for the implementation of marker-assisted selection in breeding programs and also allows advances in more studied species such as C. annuum. Considering the slowness and higher cost of the processes of isolation and specific indicators development for microsatellite analysis, the results obtained at this work allows an improvement of the genetic mapping process of C. baccatum species.