Klotho-hypomorphic (Klothohm) mice suffer from renal salt wasting and hypovolemia despite hyperaldosteronism. The present study explored the effect of Klotho on renal Na+/K+ ATPase activity. According to immunohistochemistry and confocal microscopy Na+/K + ATPase protein abundance in isolated collecting ducts was lower in Klothohm mice than in their wild type littermates (Klotho +/+). Analysis with dual electrode voltage clamp recording showed that expression of Klotho in Xenopus oocytes increased the Na+/K + ATPase pump current. Treatment of Xenopus oocytes with Klotho protein similarly increased the pump current. In conclusion, Klotho increases the membrane abundance and activity of the Na+/K+ ATPase. Decreased Na+/K+ ATPase activity could thus contribute to the volume-depletion of klothohm mice. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Sopjani, M., Alesutan, I., Dërmaku-Sopjani, M., Gu, S., Zelenak, C., Munoz, C., … Lang, F. (2011). Regulation of the Na+/K+ ATPase by Klotho. FEBS Letters, 585(12), 1759–1764. https://doi.org/10.1016/j.febslet.2011.05.021