Detection of mitochondrial biomarkers in eutopic endometria of endometriosis using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry

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Abstract

Objectives: To detect specific mitochondrial proteins in eutopic endometrial samples from women with and without endometriosis and to build diagnostic models. Design: Eutopic endometrial samples from women with endometriosis (excluding adenomyosis) and women with benign indications as control were studied by using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein-chip technology. After finding the biomarkers, the diagnostic model was evaluated and validated by leave-one cross-validation. Setting: Collaborative investigation in an academic research environment. Patient(s): Twenty-four patients with endometriosis (excluding adenomyosis) and 29 patients with benign indications as control. Intervention(s): Surgical excision of eutopic endometrial biopsy of patients with endometriosis and controls. Main Outcome Measure(s): Mitochondrial protein expression. Result(s): Seventy-eight qualified mitochondrial protein peaks were detected, ten of them had a significant difference. Three combined potential biomarkers, with mass-to-charge ratios (m/z) of 15,334, 15,128, and 16,069, were found, and the diagnostic system distinguished endometriosis from control samples with a specificity of 86.2% and a sensitivity of 87.5%. Conclusion(s): We discovered potential mitochondrial biomarkers of eutopic endometrium in endometriosis and set up a diagnostic model. Further identification of the proteins we found will help to explain pathology, new diagnoses, and therapeutic approaches for endometriosis. Copyright © 2010 American Society for Reproductive Medicine, Published by Elsevier Inc.

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APA

Ding, X., Wang, L., Ren, Y., & Zheng, W. (2010). Detection of mitochondrial biomarkers in eutopic endometria of endometriosis using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Fertility and Sterility, 94(7), 2528–2530. https://doi.org/10.1016/j.fertnstert.2010.04.054

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