Generation of human iPSCs from an essential thrombocythemia patient carrying a V501L mutation in the MPL gene

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Abstract

Activating point mutations in the MPL gene encoding the thrombopoietin receptor are found in 3%–10% of essential thrombocythemia (ET) and myelofibrosis patients. Here, we report the derivation of induced pluripotent stem cells (iPSCs) from an ET patient with a heterozygous MPL V501L mutation. Peripheral blood CD34+ progenitor cells were reprogrammed by transient plasmid expression of OCT4, SOX2, KLF4, c-MYC plus BCL2L1 (BCL-xL) genes. The derived line M494 carries a MPL V501L mutation, displays typical iPSC morphology and characteristics, are pluripotent and karyotypically normal. Upon differentiation, the iPSCs are able to differentiate into cells derived from three germ layers.

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Liu, S., Ye, Z., Gao, Y., He, C., Williams, D. W., Moliterno, A., … Cheng, L. (2017). Generation of human iPSCs from an essential thrombocythemia patient carrying a V501L mutation in the MPL gene. Stem Cell Research, 18, 57–59. https://doi.org/10.1016/j.scr.2016.12.012

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