Aim: The aim of present work was to study the pharmacognostic standardization, physicochemical, phytochemical and pharmacological evaluation of the stem of Bacopa monnieri L. Method: Standard method was followed for pharmacognostic, physicochemical, and phytochemical study. For quantitative analysis, HPTLC technique was employed. Maximal electroconvulsive shock (MES) and pentylenetetrazole (PTZ) method was used for anticonvulsant evaluation. Result: Microscopic study of the stem showed presence of epidermis and cortex having large intercellular spaces. The oval shaped starch grains were present in cells of cortex and endodermis. Total ash (5.98%), water-soluble ash (4.91%), acid-insoluble ash (3.96%), water soluble extractive (15.31%) and ethanol soluble extractive (6.74%) values were evaluated for physicochemical evaluations. Quantitative estimation of Bacoside A (marker compound) by HPTLC technique was performed. The linearity (100-600 ng), the % recovery (97.81% w/w) were estimated. The Bacoside A in the alcoholic extract was found to be higher than that of the other extracts. The proposed HPTLC method was found to be simple and accurate. The anticonvulsant activities of all the extracts were evaluated by MES and PTZ models in mice. Conclusion: The least anticonvulsant potential was recorded for acetone extract in dose of 100 mg/kg and maximum anticonvulsant action was recorded for ethanolic extract in dose of 300 mg/kg.
Mishra, A., Mishra, A. K., PrakashTiwari, O., & Jha, S. (2015). Pharmacognostic, physicochemical and phytochemical investigation of Bacopa monnieri L. stem and its anticonvulsant potential in laboratory animals. International Journal of Epilepsy, 2(1), 19–27. https://doi.org/10.1016/j.ijep.2015.02.004