Cyclin A-mediated inhibition of intra-Golgi transport requires p34cdc2

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An in vitro assay was used to study the role of p34cdc2 in cyclin A-mediated vesicular transport inhibition. It was shown that the S-phase kinase p33cdk2 reduced the effect of cyclin A on transport assays performed with sHeLa cytosol, even though histone kinase was strongly activated. Also, transport with FT210 cytosol (which is temperature-sensitive for p34cdc2) was inhibited by cyclin A only at the permissive temperature. However, the phosphatase inhibitor microcystin inhibited transport without any requirement for p34cdc2 activity. These results show that transport is inhibited by cyclin A via p34cdc2, and also by another kinase, possibly downstream of p34cdc2. © 1993.




Mackay, D., Kieckbusch, R., Adamczewski, J., & Warren, G. (1993). Cyclin A-mediated inhibition of intra-Golgi transport requires p34cdc2. FEBS Letters, 336(3), 549–554.

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