G-Protein Coupled Receptors: Experimental and Computational Approaches

Abstract

A review. This article describes the exptl. and computational approaches of G-protein coupled receptors. Guanine Nucleotide binding protein coupled receptors (GPCRs) are among the most important targets in the treatment of cancer, endocrine, neural and many other types of disorders. The 3D structures of GPCRs have been identified using different techniques such as ESR spectroscopy (EPR), site directed mutagenesis, Fluorescence spectroscopy, cysteine crosslinking studies, Atomic Force Microscopy (AFM) and X-ray crystallog. Biochem. and mutagenesis of B2AR established two major interactions for full agonists in which the amine group forms a salt bridge in AsplB while the hydrogen groups of catechol interact with serine in TM5. [on SciFinder(R)]